萌出期 永久齒 表層琺瑯質의 石灰化狀態와 表面 및 結晶構造에 關한 電子顯微鏡的 硏究
A SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC STUDY ON THE MINERALIZATION STATUS AND SURFACE AND CRYSTAL STRUCTURES OF SURFACE ENAMEL OF HUMAN PERMANENT TEETH AT PERI-ERUPTING PERIOD
소속 상세정보
김신(金伸)/Shin Kim
김진태(金鎭泰)/Jin Tae Kim
KMID : 0358919890160010085
Abstract
結論
萌出期齒牙 表層琺瑯質의 質的 成熟度를 評價하고, 口腔內 環境이 萌出後 成熟過程에 미
치는 影響을 分析하며, 琺瑯質의 表面 및 表層結晶構造를 觀察할 目的으로 萌出前 및 萌出
直後의 琺瑯質을 對象으로, 無機物 電子顯微分析과 走査形 및 透過形 電子顯微鏡을 利用한
硏究를 施行하였다.
表層 琺瑯質의 Ca, P의 含量은 電子顯微分析을 利用하여 定性, 定量分析을 試圖하고, Na
와 Mg의 含量을 線分析하여 分布狀態를 觀察하였으며, 未萌出 齒牙 琺瑯質을 口腔內에서
90日間 露出시킨 後 時間經過에 따른 含量變化를 追跡하였으며, 이 時期의 琺瑯質 表面을
觀察하고, 非脫灰琺瑯質 超薄切試片을 利用하여 結晶構造를 觀察하여 다음과 같은 結論을
얻었다.
1. 萌出을 前後하여 齒面으로부터의 깊이에 따른 Ca, P의 濃度傾斜가 反轉하고, 萌出 後
年齡 增加와 함께 含量이 增加하며, Ca/P 比率이 上昇하고, Na와 Mg의 濃度傾斜가 明確해
지는 現象이 나타났다.
2. 未萌出-琺瑯質을 90日間 露出시킨 結果, 表層 20μm 內에서 石灰化度의 有意한 上昇이
觀察되었다.
3. 萌出期 齒牙의 表層琺瑯質에서는 Tome突起孔과 小柱末端痕, 琺瑯質 頭冠狀物 및 巢狀
孔이 觀察되었으며, 萌出直後 表層琺瑯質의 機械的인 破壞像이 나타났다.
4. 透過形 電子顯微鏡을 通한 非脫灰 琺瑯質 結晶의 觀察結果, 縱橫斷된 結晶과 小柱間 空
間, 結晶間 空間, 結晶核의 形態 및 未成熟 結晶의 分布가 觀察되었다.
#초록#
With a view to evaluating the qualitative maturity of tooth enamel at peri-erupting
period, analyzing the influence of oral environment on the posteruptive enamel
maturation and observing the surface and crystal structures of this period , an electron
probe microanalyses and scanning and transmission electron microscopic study was
performed on the pre-eruptive and post-eruptive enamel of permanent teeth.
The Ca and P content in the surface layer were quantitatively and qualitatively
analyzed by Electron Probe Microanalyzer (EPMA), Energy Dispersive X-ray
Spectrometer (EDS) and Wavelength Dispersive X-ray Spectrometer (WDS). Na and Mg
concentration gradient were traced by WDS. Also the changes in Ca and P content of
pre-eruptive enamel after artificial exposure to oral environment for 90 days were
measured with WDS. Surface microstructures of the teeth in this period were observed
under Scanning Electron Microscope (SEM), and enamel crystal structures and their
arrangement were observed using undecalcified ultrathin sectioned specimen under
Transmission Electron Microscope (TEM).
The results were as follows :
1. Reversal of concentration gradient of Ca and P gradual increase in their content
and Ca/P ratio with increasing posteruptive age, and steepening of Na and Mg
concentration gradient were observed at peri-erupting period.
2. Significant increase in Ca and P content were observed within superficial 20 micron
depth by artificial oral exposure of pre-eruptive enamel for 90 days.
3. In scanning electron microscopy of pre-eruptive enamel, Tome's process pits, prism
end markings, enamel caps and focal holes were observed. Superficial destruction by
mechanical wear was also observed just after eruption.
4. The characteristic findings of surface enamel crystals of peri-erupting teeth under
TEM were ; intercrystal spaces between cross and longitudinally sectioned enamel
crystal, interprismatic spaces, cleft or mottled areas in the crystal core portions, flattened
hexagonal shaped hydroxyapatite crystals with varying diameter, and the distribution of
zone of immature crystals.
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